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FACULTY Department Members By Category Laboratories Faculty Robert Salata, MD Chief Donald Anthony, MD, PhD Keith Armitage, MD Eric Arts, PhD David Bobak, MD Robert Bonomo, MD W. Henry Boom, MD David Canaday, MD Michael Cho, PhD Curtis Donskey, MD Scott Fulton, MD Yong Gao, MD, PhD Barbara Gripshover, MD Marion Helfand, MD Christina Hirsch, MD Marta Jakubowycz, MD Wei Jiang, MD John L Johnson, MD Michael Lederman, MD Tracy Lemonovich, MD Michelle Lisgaris, MD Amy Ray, MD Louis Rice, MD Benigno, Rodriguez, MD Roxana Rojas, MD Scott Sieg, PhD Richard Silver, MD Usha Stiefel, MD Zahra Toossi, MD Gopal Yadavalli, MD Research Staff Administrative Staff SEARCH: RELATED SITES:	Robert Bonomo, MD Associate Professor of Medicine Director Cleveland VAMC GRECC Phone: (216) 991-3800 ext 4399 Fax: (216) 241-3482 E-mail: robert.bonomo@med.va.gov Education BA-Hamilton College, 1976 MD-Case Western Reserve University School of Medicine, 1983 Residecny- University Hospitals of Cleveland, 1986 Infectious Diseases, University Hospitals of Cleveland,1994 Research Among the major problems faced in medicine, resistance to antibiotics is regarded as a singular challenge. The primary focus of our laboratory is to understand the genetic and amino acid sequence determinants of the enzymes that inactivate ß-lactams, the ß-lactamases. Our current work is directed at solving three main problems: (i) how ß-lactamase inhibitors inactivate -lactamases; (ii) how ß-lactamases hydrolyze advanced generation cephalosporins; and (iii) how mutations impact upon ß-lactamase expression. We are also interested in how penicillin interacts with penicillin resistant PBPs and the molecular epidemiology of extended spectrum ß-lactamases (ESBLs). Lab Members: Andrea M. Hujer, Christopher R. Bethel Collaborators: Dr. Marion S. Helfand, Dr. Curtis J. Donskey, Dr. Louis B. Rice, Dr. David L. Paterson, Dr. Focco van den Akker, Dr. Paul R. Carey, Dr. Vernon E. Anderson, Dr. James R. Knox, Dr. Malcolm Page, Dr. Lily Ng, Dr. John Buynak, Dr. Shu Chen, Dr. Michael Kinter, Dr. Anthony Fink, Dr. John Quinn. NIH Biosketch Selected References: • Helfand MS, Hujer AM, and Sonnichsen FD, Bonomo RA. Unexpected advanced generation cephalosporinase activity of the M69F variant of SHV beta-lactamase. J Biol Chem. 2002 Dec 6; 277(49):47719-47723. • Helfand MS, Bethel CR, Hujer AM, Hujer KM, Anderson VE, Bonomo RA. Understanding resistance to beta-lactams and beta-lactamase inhibitors in the SHV beta-lactamase: lessons from the mutagenesis of Ser130. J Biol Chem. 2003 Dec 26; 278(52):52724-52729. • Pagan-Rodriguez D, Zhou X, Simmons R, Bethel, CR, Hujer AM, Helfand MS, Jin Z, Guo B, Anderson VE, Ng LM, Bonomo RA. Tazobactam inactivation of SHV-1 and the inhibitor resistant Ser130Gly SHV-1 beta-lactamase: insights into the mechanism of inhibition. J Biol Chem. 2004 Feb 6 [Epub ahead of print]. • Hujer AM, Bethel CR, Bonomo RA. Antibody mapping of the linear epitopes of CMY-2 and SHV-1 beta-lactamases. In press. Antimicrob Agents Chemother. 2004


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